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How Blunt-End Cloning Works
Manage episode 380107962 series 3391757
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#81 — You’re probably aware of the two main types of restriction cloning (sticky-end and blunt-end cloning), but do you know the difference? And do you know how to do both?
In this episode, we share what blunt-end cloning is, how it works, and give you tips and tricks for performing blunt-end cloning.
Visit the original article for detailed diagrams and helpful tables, [1] brush up on how DNA ligase works, [2] and reacquaint yourself with the different DNA polymerases. [3] You can also get help to improve your blunt end ligations, [4] and discover the magic of TA cloning. [5]
Resources:
1. A Beginner’s Guide to How Blunt-End Cloning Works. Available at: https://bitesizebio.com/45131/a-beginners-guide-to-how-blunt-end-cloning-works/
2. DNA Ligation: How it Works & 6 Top Tips. Available at: https://bitesizebio.com/10279/the-basics-how-does-dna-ligation-work/
3. Get To Know Your DNA Polymerases. Available at: https://bitesizebio.com/24551/get-to-know-your-dna-polymerases/
4. 10 ways to improve blunt-end ligations. Available at: https://bitesizebio.com/19149/7-ways-to-improve-blunt-end-ligations/
5. Ta-Da! The Magic of Taq and TA Cloning. Available at: https://bitesizebio.com/19709/ta-da-the-magic-of-taq-and-ta-cloning/
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